glt 1 inhibitor way 213613  (MedChemExpress)

Journal: PLoS ONE

Article Title: Differential Regulation of Glutamate Transporter Subtypes by Pro-Inflammatory Cytokine TNF-α in Cortical Astrocytes from a Rat Model of Amyotrophic Lateral Sclerosis

doi: 10.1371/journal.pone.0097649

Figure Lengend Snippet: Glutamate transporter activity was evaluated by measuring velocity of d -[ 3 H]-aspartate uptake (50 nmol/L) in wild-type and hSOD1 G93A astrocytes treated or not with TNF-α (20 ng/mL) for 72 h. (A) shows total uptake while (B) and (C) illustrate GLT-1- and GLAST-dependent uptake, respectively evaluated in presence of the selective inhibitors WAY-213613 (100 µmol/L) and UCPH-101 (10 µmol/L). Shown are mean with SEM from five independent experiments realized in quintuplicate. # p <0.05 and ### p <0.001 for comparison between different genotypes with the same treatment.

Article Snippet: Inhibitors of GLT-1 (WAY-213613, Tocris, Bristol, UK) and GLAST (UCPH-101, Tocris) were used at 100 and 10 μmol/L, respectively.

Techniques: Activity Assay, Comparison